Development and Validation of Competitive ELISA for Detection of H5 Hemagglutinin Antibodies

نویسندگان

چکیده

Influenza A viruses (IAVs) belonging to the goose/Guangdong (Gs/GD)-lineage H5Nx remain a major concern for global poultry industry, wildlife, and humans. The hemagglutinin (HA) protein is dominant antigenic epitope carrier within IAV, which in turn triggers substantial immunogenic responses infected host. current study describes development validation of highly sensitive competitive H5 ELISA (cELISA) based on novel monoclonal antibody developed mice immunized with inactivated virus H5N1 (A/Turkey/ON/6213/1966). cELISA capable detecting response wider range H5-subtype both North American Eurasia lineages, including Gs/GD from clade 2.3.4.4b that currently causing pathogenic avian influenza outbreaks Eurasia, Africa, Latin America. provides specific, sensitive, species-independent serological assay rapid detection antibodies. more robust than hemagglutination inhibition assay, “Gold standard”. can be used diagnosis, serosurveillance, vaccine monitoring serum samples collected different species animals.

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ژورنال

عنوان ژورنال: Poultry

سال: 2023

ISSN: ['2674-1164']

DOI: https://doi.org/10.3390/poultry2030026